The 45-year-old made everyone stand up and took notice as she strutted out on the Hollywood Bowl stage in Los Angeles, California, on Friday night for the We Can Survive concert.
The singer stepped out in the middle of Iggy Azalea’s set to perform their hit Booty and turned it into a interpretive dance of the rather saucy lyrics.
Not wasting any time, the music icon playfully spanked her Australian counterpart’s derriere much to the amusement of the crowd.
But JLo was not done there, with the I Luh Ya Papi twerking and dipping her way through the song.
Even without the sexy dance moves, Jennifer would have set temperatures soaring thanks to her very sexy ensemble.
For her surprise performance, the 45-year-old wore a pair of high waisted silver lame booty shorts with a matching bra that showed off her impressive physique.
Keeping with her theme, the star added a pair of fishnet stockings and knee high silver gladiator heels.
Perhaps taking her lead from fellow We Can Survive performer, Ariana Grande, JLo wore her hair up in a high ponytail with lots of voluminous hair extensions.
Speaking before her performance to CBS Radio Iggy gushed about her fellow performers.
A little star struck the 24-year-old said: ‘So many of these people, almost all of these people, I grew up being massive fans of, particularly Pharrell and Gwen and JLo.’
Short shorts were certainly the must have item of the huge concert, with Taylor Swift also rocking a pair.
The 24-year-old star somehow managed to embrace both sweet and sexy, wearing an all-white look.
The I Knew You Were Trouble made her long thin legs the centre of attention in a pair of simple high waisted shorts.